Incorporation of Thymine into Prototrophic and Thymine-dependent Mutants of Bacillus anthracis
نویسندگان
چکیده
منابع مشابه
Uracil incorporation into nascent DNA of thymine-requiring mutant of Bacillus subtilis 168.
A thymine-requiring mutant of Bacillus subtilis strain 168 accumulates short DNA chains after brief pulses with [(3)H]thymidine. Reversion of the thy mutation to thy(+) abolishes the accumulation of short DNA chains, suggesting that the accumulation is related to the thy mutation. The reason for this accumulation has been further investigated by analysis of a mutant with a defective uracil-DNA ...
متن کاملMechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase eta.
DNA polymerase eta (Poleta) has the unique ability to replicate through UV-light-induced cyclobutane pyrimidine dimers. Here we use pre-steady-state kinetic analyses to examine the mechanism of nucleotide incorporation opposite a cis-syn thymine-thymine (TT) dimer and an identical nondamaged sequence by yeast Poleta. Poleta displayed "burst" kinetics for nucleotide incorporation opposite both t...
متن کاملUptake and Incorporation of Thymine, Thymidine, Uracil, Uridine, and 5-fluorouracil into the Nucleic Acids of Bacillus Subtilis.
Bodmer, Walter F. (Stanford University School of Medicine, Palo Alto, Calif.), and Susan Grether. Uptake and incorporation of thymine, thymidine, uracil, uridine, and 5-fluorouracil into the nucleic acids of Bacillus subtilis. J. Bacteriol. 89:1011-1014. 1965.-From 55 to 95% of uracil, uridine, or 5-fluorouracil (FU) added to the culture medium is incorporated into the acid-insoluble fraction o...
متن کاملIncorporation of labelled degradation products of radioactive thymine into non DNA material.
When thymine auxotrophs are grown in the presence of methyl labelled [3H] or [14C] thymine which has been stored at 4 degrees C, two classes of material are labelled which are not DNA. One class sediments on neutral sucrose gradients with spontaneously single stranded Okazaki pieces, is unstable in alkali, migrates on alkaline gels as very small material and is digested by ribonucleases and mic...
متن کاملConversion of uracil to thymine by strains of bacillus subtilis.
From replacement studies (1) and inhibition analysis (2) with microorganisms requiring folic acid, it has been inferred that this vitamin is concerned in the biosynthesis of purines and of thymine. More directly, folic acid has been shown to be involved in nucleic acid synthesis in Lactobacillus casei (3,4). According to Buchanan and Schulman (5), the formation of purine derivatives would seem ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1969
ISSN: 0022-1287
DOI: 10.1099/00221287-59-3-337